- 394 ：名無しゲノムのクローンさん：2014/02/20(木) 18:52:44.65
- Here we report a unique cellular reprogramming phenomenon,
called stimulus-triggered acquisition of pluripotency (STAP),
which requires neither nuclear transfer nor the introduction of
transcription factors. In STAP, strong external stimuli such as
a transient low-pH stressor reprogrammed mammalian somatic cells,
resulting in the generation of pluripotent cells.
Through real-time imaging of STAP cells derived from purified lymphocytes,
as well as gene rearrangement analysis, we found that committed somatic cells
give rise to STAP cells by reprogramming rather than selection.
STAP cells showed a substantial decrease in DNA methylation
in the regulatory regions of pluripotency marker genes.
Blastocyst injection showed that STAP cells efficiently contribute to
chimaeric embryos and to offspring via germline transmission.
We also demonstrate the derivation of robustly expandable pluripotent cell lines
from STAP cells. Thus, our findings indicate that epigenetic fate determination of
mammalian cells can be markedly converted
in a context-dependent manner by strong environmental cues.
read.cgi ver 05.04.00 2017/10/04 Walang Kapalit ★
FOX ★ DSO(Dynamic Shared Object)